Key role for ceramides in mediating insulin resistance in human muscle cells
L Pickersgill, GJ Litherland, AS Greenberg… - Journal of Biological …, 2007 - ASBMB
Elevated non-esterified fatty acids, triglyceride, diacylglycerol, and ceramide have all been
associated with insulin resistance in muscle. We set out to investigate the role of
intramyocellular lipid metabolites in the induction of insulin resistance in human primary
myoblast cultures. Muscle cells were subjected to adenovirus-mediated expression of
perilipin or incubated with fatty acids for 18 h, prior to insulin stimulation and measurement
of lipid metabolites and rates of glycogen synthesis. Adenovirus-driven perilipin expression …
associated with insulin resistance in muscle. We set out to investigate the role of
intramyocellular lipid metabolites in the induction of insulin resistance in human primary
myoblast cultures. Muscle cells were subjected to adenovirus-mediated expression of
perilipin or incubated with fatty acids for 18 h, prior to insulin stimulation and measurement
of lipid metabolites and rates of glycogen synthesis. Adenovirus-driven perilipin expression …
