Proximal Tubule–Derived Amphiregulin Amplifies and Integrates Profibrotic EGF Receptor Signals in Kidney Fibrosis
E Kefaloyianni, MRK Raja, J Schumacher… - Journal of the …, 2019 - journals.lww.com
Background Sustained activation of EGF receptor (EGFR) in proximal tubule cells is a
hallmark of progressive kidney fibrosis after AKI and in CKD. However, the molecular
mechanisms and particular EGFR ligands involved are unknown. Methods We studied
EGFR activation in proximal tubule cells and primary tubular cells isolated from injured
kidneys in vitro. To determine in vivo the role of amphiregulin, a low-affinity EGFR ligand that
is highly upregulated with injury, we used ischemia-reperfusion injury or unilateral ureteral …
hallmark of progressive kidney fibrosis after AKI and in CKD. However, the molecular
mechanisms and particular EGFR ligands involved are unknown. Methods We studied
EGFR activation in proximal tubule cells and primary tubular cells isolated from injured
kidneys in vitro. To determine in vivo the role of amphiregulin, a low-affinity EGFR ligand that
is highly upregulated with injury, we used ischemia-reperfusion injury or unilateral ureteral …
